|
KMID : 0380020060210050366
|
|
Korean Journal of Biotechnology and Bioengineering
2006 Volume.21 No. 5 p.366 ~ p.370
|
|
|
Effect of Nitrogen Source on the Change of UDP-glucose and β-1,3-glucan Concentration
|
|
Park Yang-Ho
Lee Jung-Heon
|
|
|
Abstract
|
|
|
|
In this research, analysis of UDP-glucose a precursor of β-1,3-glucan by high performance liquid chromatography (HPLC) was established using a reversed phase system. One of key metabolite UDP-glucose was selected and its concentration changes was measured with the change of fermentation conditions. The effects of fermentation conditions with/without nitrogen source for cell growth on β-1,3-glucan production were dependent on the UDP-glucose concentration. The UDP-glucose was synthesized rapidly during cell exponential growth period and maintained high during β-1,3-glucan production period. The UDP-glucose concentration was higher for β-1,3-glucan production fermentor than that for cell growth fermentor. The β-1,3-glucan production was optimal at pH 5.5 and synthesis of β-1,3-glucan was greatest at pH 5.5.
|
|
|
KEYWORD
|
|
|
β-1, 3-glucan, UDP-glucose, nitrogen source
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|